anti cd16 primary antibody  (Bioss)

Journal: PLoS ONE

Article Title: IL-15 Deficient Tax Mice Reveal a Role for IL-1α in Tumor Immunity

doi: 10.1371/journal.pone.0085028

Figure Lengend Snippet: A) Incidence of tumor onset in IL-15 −/− TAX-LUC mice (red line, n = 10) compared to IL-15 +/+ TAX-LUC littermate control mice (black line, n = 21), p = 0.03 (2-tailed, paired Student’s T Test). B) Survival curve comparing IL-15 −/− TAX-LUC mice (red line, n = 13) and IL-15 +/− TAX-LUC mice (blue line, n = 14) to IL-15 +/+ TAX-LUC littermate control mice (black line, n = 10). p = 0.006 for KO vs. WT and p = 0.02 for HET vs. WT. C) Image of CD16/32 immunohistochemistry of IL-15 −/− TAX-LUC tumor sections in which the malignant large granular lymphocytes (red arrows) are stained along with an admixture of tumor infiltrating neutrophils (black arrows). Insets are FACS histograms of homogenates from IL-15 +/+ TAX-LUC or IL-15 −/− TAX-LUC tail tumors unstained (white curves) or stained with anti-CD16/32 FITC (red curves). D) Image of Ly6G immunohistochemistry of IL-15 −/− TAX-LUC tumor sections in which tumor infiltrating neutrophils (black arrows) are stained. Insets are FACS histograms of homogenates from IL-15 +/+ TAX-LUC or IL-15 −/− TAX-LUC tail tumors unstained (white curves) or stained with anti-Ly6G-PE (red curves). E) Image of phosho-RelA immunohistochemistry of IL-15 −/− TAX-LUC tumor sections in which many of the malignant large granular lymphocytes (red arrows) show nuclear localization as a marker of NFκB activation. F) Image of a peripheral blood smear (Wright’s stain). G) Hematoxylin and eosin stained IL-15 −/− TAX-LUC tumor section. H) Image of Ki67 immunohistochemistry of IL-15 −/− TAX-LUC tumor sections.

Article Snippet: For CD16/32 staining additional blocking steps with avidin D solution (Vector Laboratories) and for 15 min at room temp, followed by biotin for 15 minutes at room temperature was included prior to incubation in primary biotin rat anti-mouse CD16/32 antibody (BD Pharmingen) overnight at 4°C.

Techniques: Immunohistochemistry, Staining, Marker, Activation Assay

Journal: PLoS ONE

Article Title: IL-15 Deficient Tax Mice Reveal a Role for IL-1α in Tumor Immunity

doi: 10.1371/journal.pone.0085028

Figure Lengend Snippet: A) Schematic representation of experimental design. RNA harvested from CD16/32 HI and CD16/32 LO cells from IL-15 +/+ and IL-15 −/− TAX-LUC tail tumors were analyzed by array. B) Profiles of gene expression changes associated with the absence of IL-15 in CD16/32 HI and CD16/32 LO tumor cell populations.

Article Snippet: For CD16/32 staining additional blocking steps with avidin D solution (Vector Laboratories) and for 15 min at room temp, followed by biotin for 15 minutes at room temperature was included prior to incubation in primary biotin rat anti-mouse CD16/32 antibody (BD Pharmingen) overnight at 4°C.

Techniques: Expressing

Journal: PLoS ONE

Article Title: IL-15 Deficient Tax Mice Reveal a Role for IL-1α in Tumor Immunity

doi: 10.1371/journal.pone.0085028

Figure Lengend Snippet: A) Images of IL-1α IHC in IL-15 +/+ (middle) and IL-15 −/− (right) TAX-LUC tumor sections compared to control lacking primary IL-1α antibody (left). B) The effect of anti-IL-1α antibodies (red curve, n = 5) on tumor growth compared to saline (black curve, n = 5) in IL-15 +/+ TAX-LUC mice over the course of 1 week. Error bars represent standard deviation of tumor growth, and p values represent 2-tailed, paired Student’s T Tests. The bar graph (right) represents FACS data obtained from anti-IL-1a treated tumors (red bars) or control tumors (black bars) at necropsy showing the abundance of CD8 + , CD4 + , CD16/32 HI , and CD16/32 LO cells present in the tumor at the experimental endpoint. Y axis is log scale and error bars represent standard deviation. C) The effect of Anakinra on tumor growth compared to saline. In 7 of 9 mice Anakinra resulted in repression of tumor growth (Anakinra R, red line), while in 2 of 9 mice Anakinra resulted in promotion of tumor growth (Anakinra P, blue line). Graph is double-Y to accommodate the scale differences between Anakinra R and control (left scale) versus Anakinra P (right scale). Red arrows indicate the time points of daily Anakinra injections, error bars represent standard deviations and p values represent 2-tailed, paired Student’s T Tests. The bar graph (right) represents FACS data obtained from Anakinra treated tumors or control tumors at necropsy showing the abundance of CD8 + , CD4 + , CD16/32 HI , and CD16/32 LO cells present in the tumor at the experimental endpoint. Y axis is log scale and error bars represent standard deviation.

Article Snippet: For CD16/32 staining additional blocking steps with avidin D solution (Vector Laboratories) and for 15 min at room temp, followed by biotin for 15 minutes at room temperature was included prior to incubation in primary biotin rat anti-mouse CD16/32 antibody (BD Pharmingen) overnight at 4°C.

Techniques: Standard Deviation